Abstract

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANIOUS ESTIMATION OF ATTAZANAVIR AND COBICISTAT IN TABLET DOSAGE FORM

Nagaraju Perabathina*, Paul Richards M., Chandra Sekhar K.B., Kumar Sigamala

ABSTRACT

A simple sensitive, and precise high performance liquid chromatographic method for the analysis of atazanavir and cobicistat hasbeen developed and validated for the simultaneous determination of copounds in commercial pharmaceutical products. The compounds were well separated on ODS intersil C18 reverse phase column by the use of mobile phase of Orthophosphoric acid acetonitrile in a ratio of 45:55 v/v at a flow rate of 1.0 ml/min with detection wavelength at 270 nm.The retensioin time of atazanavir and cobicistat was found to be 4.135min and1.668min The method was validated in terms of leniarity, precision, accuracy, and specificity, robustness, ruggedness and solution stability Degradation studies like acid, base, peroxide, thermal, uv and water. The method dies require only 10 min as run time for analysis which prove the adoptability of the method for the routine quality control analysis of the drug.

Keywords: Atazanavir and Cobicistat, RP-HPLC.