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Abstract

PHARMACOGNOSTIC STUDY, CHARACTERIZATION OF MARKER COMPOUNDS AND PHARMACOLOGICAL REVIEW OF AERIAL PARTS OF HYGROPHILA AURICULATA (SCHUMACH.) HEINE

Dr. Vijay Nigam*, Dr. Rajesh Kumar Mishra, Ankita Gupta and Dr. Manoj Kumar Bhati

ABSTRACT

Hygrophila auriculata belonging to family Acanthaceae; is also known as Asteracantha longifolia and commonly known as “Neermulli, Talmakhana, Kokilaksha & Iksura” is a common plant growing in marshy and water logged areas. The plant is an important medicinal herb, widely distributed in India and is used for different medicinal purposes. Asteracantha longifolia (L.) Nees, Acanthaceae, is a source of the Ayurvedic drug, 'Kokilaaksha' and the Unani drug, Talimakhana. The aerial parts of the plant are acrid, bitter, aphrodisiac, tonic, sedative, used for blood disorders. The plant is known to possess antitumor, hypoglycemic, aphrodisiac, antibacterial, free radical scavenging and lipid peroxidation, hepatoprotective and haematopoietic activity. It contains lupeol, stigmasterol, butelin, fatty acids, and alkaloids. It is also used commercially as an ingredient of some over the counter (OTC) formulations in liver disorder and those prescribed by general tonic. Many constituents have been reported from the plant Asteracantha longifolia. In the present study extracted aerial parts of Asteracantha Asteracantha longifolia were subjected to isolation and purification of phytoconstutients as marker. Isolation of phytoconstutients was done by Column chromatography using gradient elution with different mobile phases and silica gel as stationary phase. Petroleum Ether fractions were subjected to Gas chromatography to isolate fatty acids and lipids. Four fatty acids were isolated by gas chromatography. The compounds are Palmitic acid, Stearic acid, Oleic acid and Linoleic acid respectively.

Keywords: Asteracantha longifolia, Marker compounds, Column chromatography, GC, IR, NMR, MASS spectroscopy, Antitumor, Hypoglycemic, Aphrodisiac, Antibacterial, Free radical scavenging and Lipid peroxidation, Hepatoprotective and Haematopoietic activity.


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