Abstract

EVALUATION OF NON-ENZYMATIC AND ENZYMATIC ANTIOXIDANTS ACTIVITY IN FRUIT PEELS

Akhilesh Bind*, Archana Singh, Veeru Prakash, Nabeel Ahmad and Manish Kumar

ABSTRACT

Three species of some selected fruits were chosen to investigated and evaluate the level of non-enzymatic and enzymatic antioxidants in peels of Citrus sinensis, Citrus limetta risso and Punica granatum; also to assess free radical scavenging activity of above mentioned fruits species by DPPH scavenging activity. The results indicate that non-enzymatic antioxidants carotenoids and total phenols were highest in peels of Citrus limetta risso (29.05+0.21 μg/ml and 16.92+1.5 mg GAE respectively). Flavonoid content was highest in peel of Punica granatum (53.09+1.24mg qurcetin equivalent per ml) and ascorbic acid content was highest in peels of Citrus sinensis (54.30+0.004mg/100g). The specific activity (U/mg protein) of enzymatic antioxidant viz, superoxide acid oxidase were highest in peels of Punica grantum (0.369+0.003,31.24+0.02 U/mg protein respectively), catalase and polyphenol oxidase were highest in peel of Citrus sinensis(43.7+0.82 and 12+1.08 U/mg) protein respectively. Ethanolic extracts of these two fruits viz. Punica granatum and Citrus limetta risso fruit peels exhibited maximum antioxidants activity except in peels of Citrus sinensis that showed best antioxidant activity in chloroform extract.

Keywords: Antioxidants, Citrus sinensis, Punica granatum,Citrus limetta risso.