Abstract

APPLICATION OF CIPROFLOXACIN AS A FLUORESCENCE PROBE FOR THE DETERMINATION OF ACETAMINOPHEN IN PHARMACEUTICAL TABLET

Qian Wang, Ming Su, Peiyun Chen, Hanwen Sun*

ABSTRACT

A new method using ciprofloxacin as a fluorescence probe for determination of acetaminophen in the tablet was developed. The inhibition effect of acetaminophen on fluorescence of ciprofloxacin was discussed. Fluorescence emission conditions such as pH and dosages of B-R buffer solution, and ciprofloxacin concentration, and reaction time, were investigated and optimized. Interfering effects of some excipients and potentially interfering ions on fluorescence intensity were investigated. Under the optimized conditions, calibration curve for the quenching value of fluorescence intensity toward acetaminophen concentration was linear, with a correlation coefficient (r) of 0.996. The LOD of 2.5×10–7 g/mL for acetaminophen was achieved. The recovery for tablet analysis was 88. 4–96.9% with the RSD (n=5) of 0.78–2.0 %. This method was rapid, simple, and sensitive for the analysis of paracetamol tablet.

Keywords: Fluorescence probe, Ciprofloxacin, Acetaminophen, pharmaceutical tablet.