Abstract

INDEGENOUS OXALATE DEGRADING LACTIC ACID BACTERIA

Tram Pham, Anh Le, Nguyen Ngo and Tu Nguyen*

ABSTRACT

Lactic acid bacteria (LAB) have the potential to degrade intestinal oxalate, and this is increasingly being studied as a promising probiotic solution to manage kidney stone disease. In this study, a screening for oxalate degrading abilities was initially carried on within Lactic Acid Bacteria cultures. Eight strains (Lactobacillus fermentum, Lactobacillus salivarus, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus plantarum, Pediococcus pentosus, Lauconostoc dextranicum) were drop-inoculated onto MRS agar plates containing calcium oxalate with three different concentration of bacteria and three different time of incubation. All eight strains could degrade oxalate. Eleven strains were used to inoculate in MRS modified by potassium oxalate addition with 4 different concentrations of glucose. Differences in the oxalate degrading were appeared when quantified the amounts of potassium oxalate after incubation. All the strains developed strongest at 2% concentration of glucose and at 48 hours of incubations, so this is the best condition for lactic acid bacteria metabolized oxalate. L. crispatus, Pediococcus pentosus, Lactobacillus fermentum, Lactobacillus saliva, Lactobacillus rhamnosus, Lactobacillus acidophillus showed high oxalate degrading ability at 200 µL (2x107 cfu) of concentration and at 48 hours but Lactobacillus plantarum and Leuconostocs dextranicum were high oxalate degrading ability at 100 µL (107 cfu) at 24 hours incubation. This difference was due to the source that the bacteria were isolated. L. crispatus, Pediococcus pentosus, Lactobacillus fermentum, Lactobacillus saliva, Lactobacillus rhamnosus, Lactobacillus acidophillus had the oxalate degrading ability increasing from 24 hours to 48 hours and decreasing from 48 hours to 72 hours. The proteins (OxO, ODC) which might be in crude extracellular due to the positive degradation. The results could be an excellent foundation for the development of a safe, probiotic culture in reducing oxalates and treating kidney stones.

Keywords: Oxalate, Lactic acid bacteria, probiotic culture.