Abstract

DEVELOPMENT AND VALIDATION OF GREEN SPECTROSCOPIC METHOD FOR DETERMINATION OF ZINC SULPHATE IN TABLET DOSAGE FORM

Haifa S. Said*, Esra A. Hassan, Hijra A. Abdallah, Maab I. Abdelwahab, Shahd A. Mohamed, Amira G. Saad, Mohamed Ibrahim

ABSTRACT

Green chemistry is defined as the attempt to reduce or avoid the side effect and environmental effect of any chemical activity. A green spectrophotometric method had been developed and validated to determine zinc sulphate in dosage form based on the complexation reaction between zinc ion and polyphenols in green tea extract in acetate buffer medium (pH 4.8); the absorbance of the complex was measured at 400nm. The developed method obey to beer-lambert law in the concentration range from 3.3×103 to 13×103 μg/ml for Zn (II) with correlation coefficient 0.9995, limit of detection 9.1×102 μg/ml, and limit of quantification 2.8×103 μg/ml. The method was found to be excellent green with analytical eco-scale 92 and was applied successfully for pharmaceutical dosage form.

Keywords: Green chemistry, zinc sulphate, green tea, complex, validation.