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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPY METHOD FOR THE ESTIMATION OF CLOBETASOL PROPIONATE IN BULK AND FORMULATION

Shrikant Balaganure*, Deepak Bhosale, Amaan Shaikh and Shivprasad Patil

ABSTRACT

The spectroscopic method was developed for the estimation of clobetasol propionate in bulk and Formulation. The UV spectrum of Clobetasol propionate in Acetonitrile showed λ max at 240nm. Beer’s law is valid in the concentration range of 2.5-12.5μg/ml. This method was validated for linearity, accuracy, precision, LOD, and LOQ. The method has demonstrated excellent linearity over the range of 2.5-12.5μg/ml with regression equation y = 0.0668x - 0.0253 and regression correlation coefficient r2= 0.998. Moreover, the method was found to be highly sensitive with LOD (0.71μg/ml) and LOQ (2.16μg/ml). Depending on the results the given method can be successfully applied for the assay of clobetasol propionate in the formulation.

Keywords: UV spectroscopy, method development, and validation, Formulation, clobetasol propionate.

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