Abstract

UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF OXICONAZOLE IN BULK AND SEMISOLID DOSAGE FORM

Amaan Shaikh*, Deepak Bhosale, Shrikant Balaganure and Shivprasad Patil

ABSTRACT

A new, simple, sensitive, precise, and reproducible UV spectroscopic method was developed for the estimation of Oxiconazole nitrate in bulk and Semisolid Formulation. The UV spectrophotometric method was developed and validated for quantitative estimation of Oxiconazole. Oxiconazole is soluble in acetonitrile, methanol, and a mixture of methanol and ethanol in a ratio of 70:30v/v. Oxiconazole was dissolved in the mixture of methanol and ethanol (70:30) and the resulting solution was scanned in the UV range (200-400nm). The λmax was found to be 220nm. The concentration range for analysis was 5.5-7.5μg/ml. The developed method was validated for linearity, accuracy, precision, robustness, LOD, and LOQ. Linearity was obtained in the range of 5.5-7.5μg/ml and the regression coefficient was found to be 0.9943. LOD and LOQ were found to be μg/ml and μg/ml, respectively. The method was reproducible with accuracy. Hence, can be used for routine analysis of Oxiconazole.

Keywords: UV spectroscopy, method development, and validation, RO Water, Semisolid Formulation, Oxiconazole nitrate.