Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS JANUARY ISSUE PUBLISHED
  • JANUARY 2021 Issue has been successfully launched on 1 January 2021.

Abstract

A REVIEW ON METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC METHOD

S. Yuvasri*, S. Murugan and T. Vetrichelvan

ABSTRACT

High performance liquid chromatography (HPLC) is an analytical tool in appraising drug product. It is able to detect, separate and quantify the drug, impurities and drug related degradants that can form manufacturing and storage. Many chromatographic parameters were evaluated in order to optimize the method like sample pretreatment, choosing mobile phase, column and detector selection. HPLC method development depends upon chemical structure, synthetic route, solubility, polarity, pH, pka and functional group activity etc. It also involves the understanding of chemistry of drug substance and facilitates the development of the analytical method. This article mainly focuses on the optimization of HPLC conditions. A sequence of events required for method development and analytical validation are described. Validation of HPLC method as per ICH guidelines covers all performance characteristics like accuracy, precision, specificity, linearity, range, limit of detection, limit of quantification. All validation parameters are used in the analysis.

Keywords: RP-HPLC, instrumentation, method development, validation.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More