Abstract

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-A REVIEW

Bharati R. Hole*, Annasaheb S. Gaikwad, Ankita A. Giramkar and
Dr. Dipak G. Umalkar

ABSTRACT

Chromatography is defined as a set of techniques which is used for the separation of constituents in a mixture. This technique involves two phases’ stationary and mobile phases. High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological samples. In these chromatography techniques, HPLC is one of the chromatographic techniques, which is mostly used analytical technique. Analytical Chemistry is the branch of Science that uses advance technologies in determining the composition by analytical technique. These strategies are fundamental for various purposes, including testing for quality control discharge, testing of solidness tests, testing of reference materials and to give information to bolster determinations. It is the most versatile, safest, dependable and fastest chromatographic technique for the quality control of drug components. This review article aims to focus on the different aspects of HPLC technique its principle, types, instrumentation and applications.

Keywords: High Performance Liquid Chromatography, Mobile phase, Stationary phase.