Abstract

IMMUNOMODULATORY POTENTIAL OF THEVETIA PERUVIANA IN ANIMAL MODEL: AN IN-VIVO STUDY

Malti Kushwaha*, Sandesh Asati and Vinod Kumar Chandel

ABSTRACT

The aim of this research work to investigate the in-vivo immunomodulatory potential of Thevetia peruviana in animal model. The immunomodulatory activities of various organic leaf extracts of Thevetia peruviana was explored. Investigate begin with the phytochemical screening along with the immunomodulatory activity with selected model as Hemagglutination test (Humoral immunity) and delayed type hypersensitivity test (Cellular immunity). A high level of total phenols is a positive indication for the antioxidant properties of Thevetia peruviana. Immunomodulatory agents of plant and animal origin enhance the immune responsiveness of an organism against a pathogen by activating the immune system. However these agents and the polyhedral formulations should be subjected to systematic studies to substantiate the therapeutic claims made with regard to their clinical utility. Ethanolic extracts of Thevetia peruviana exhibited potent increase in paw oedema in response to SRBC. The initial response in the first 4 hour resulted in the infiltration of CD4 lines of T lymphocytes. The present observations appear to give some support to the use of ethanolic extract of Thevetia peruviana in traditional medicine for the treatment of autoimmune diseases. The extracts not only produced a non-specific immune response, but also improved humoral as well as cell-mediated immunity effectively. Thus, from the results obtained, it can be concluded that ethanolic extract of Thevetia peruvianahas therapeutic potential and could be served as a safe and effective immunomodulatory agent in the treatment of immunological disorders.

Keywords: Thevetia peruviana; Ethanolic extract; Immunomodulatory; Hemagglutination test; Cellular immunity.