Abstract

BURNS HEALING RATES IN RATS MEDICATED WITH LEAF EXTRACT OF TEKELAN (CHROMOLAENA ODORATA L.) OINTMENT

Ramadhan Triyandi, Muhammad Iqbal, Muhammad Fitra Wardhana S., Dwi Aulia Ramdini, Vadiyani Fricillya Puteri, Afna Nur Afni Palogan, Fredison, Nur Amrillah and Mohammad Kanedi*

ABSTRACT

Tekelan (Chromolaena odorata L.) is a type of plant that is known to contain antioxidants, anti-inflammatory, and antimicrobial properties that allow this plant to be used as a wound healing medicine, including burns. This study aims to determine the healing effect of ethanol fraction of tekelan leaf extract on wistar rats (Rattus norvegicus). By using completely randomized design five different ointments were prepared and given to five groups of experimental animals consisted of three rats each. To the each group, the treatments given are as follows: silver sulfadiazine cream (+ control), vaseline flavum (–control), tekelan extract of 5%, 10% and 15% respectively (as the test groups). Burn wound is inflicted by placing a metal rod (12 mm diameter) heated to 100 oC in boiling water on the back skin of rats that had been previously shaved. Treatment was given once daily for 21 days. Observation of wound healing, based on percent closure wound area, was carried out on day 0 (immediately after the injury), day 7, 14 and 21. The results showed that all treatments could completely heal burns in test animals within 21 days. The only difference was seen in the weekly progress rate of wound closure, where only the tekelan extract with the highest concentration (15%) nearly matched the standard drug.

Keywords: burns, wound, tekelan, Chromolaena ordorata, tekelan extract ointment.