Abstract

ANALYTICAL METHOD DEVELOPED FOR THE SIMULTANEOUS BIOGENIC AMINE QUANTIFICATION OF ADRENALINE AND NORADRENALINE

Naga Madhusudana Rao Chadaram* S. Ashutosh Kumar, Manidipa Debnath,N. Sai Krishna, J. Saravanan, V. Greeshma

ABSTRACT

Adrenaline (Epinephrine) and nor-adrenaline (nor-epinephrine) are major endogenous catecholamines which are known as neurotransmitters. This work provides a new simple, accurate, precise and reproducible an analytical method developed for the simultaneous biogenic amine quantification of adrenaline and nor-adrenaline. Chromatographic techniques are changing with improvements in sample preparation procedures, column technology and more specific analyte detection using tandem mass spectrometry. An assay of noradrenaline, adrenaline based on high-performance liquid chromatography (HPLC).The related substances were resolved on a Eurospher 100-5 C18 column (150mm × 4.6 mm, 5μm) using a mobile phase composed of a mixture of formic acid and 50mM ammonium formate buffer pH 3.0 at a flow rate of 0.8ml/min with UV detector at 266 nm. The method is compatible with HPLCMS and provides a tool for the control of substandard and counterfeit commercial preparations of the adrenaline and nor-adrenaline.

Keywords: Nor adrenaline, Adrenaline, HPLC