Abstract

SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE AND GLIMEPIRIDE BY UV-VISIBLE SPECTROSCOPY

*Kanchan R. Ghode, Dr. Manjusha P. Yeole, Dr. Dinesh Chaple, Farhin Shiekh, Shubhangi Choudhari and Srushti Wairagade

ABSTRACT

The simple, accurate, economical and reproducible spectrophotometric methods for simultaneous estimation of two-component drug mixture of Metformin hydrochloride and Glimepiride in bulk drug and pharmaceutical preparations have been developed. Developed methods are based on direct estimation of Metformin hydrochloride at 237 nm and Glimepiride at 228 nm. For estimation first developed method involves formation and solving of simultaneous equation. The developed methods obey Beer’s law in the concentration ranges 2-50μg/ml for Metformin hydrochloride and 2-50μg/ml for Glimepiride. The method validated according to ICH guidelines in respect of precision, accuracy, sensitivity and linearity.

Keywords: Simultaneous Spectrophotometric Analysis, Metformin hydrochloride, Glimepiride.