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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript

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New Impact Factor

WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

Scope Indexed

WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

WJPPS: APRIL ISSUE PUBLISHED

April Issue has been successfully launched on 1 April 2024.

Abstract

RELATED SUBSTANCE METHOD DEVELOPED AND VALIDATED FOR L-ASPARAGINASE AND VINCRISTINE BY RP-HPLC

Dr. Mannam Subba Rao* and Gopinath Kadari

ABSTRACT

A novel, simple and precise RP-HPLC method has been developing for the quantitative analysis of L- Asparaginase and Vincristine using Luna phenyl hexyl 150x4.6mm, 5μ column with a flow rate of 1ml/min. The buffer of 1ml Ortho Phosphoric Acid dissolved in 1lt of water and the mobile phase containing a mixture of 0.1% OPA and Acetonitrile in 70:30 ratio. The detection was carried out at 216nm. Method validation is carried out according to ICH guidelines and the parameters namely precision, accuracy, specificity, stability, robustness, linearity, limit of detection and limit of quantification are evaluated and the values are found to be within the acceptable limit.

Keywords: RP-HPLC, L-Asparaginase, Vincristine.

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