Abstract

DEVELOPMENT AND EVALUATION OF LIPOSOMAL DRUG DELIVERY SYSTEM CONTAINING ETOPOSIDE

Pragati Mahajan*

ABSTRACT

Reverse phase evaporation and ethanol injection, the two most popular preparative techniques, were utilized to create cationic liposomes made of etoposide API, DMPG-Na polymer, and cholesterol binder, in that order. In order to get around the drawbacks of the documented HPLC analytical method in the pharmacopeia— which is somewhat laborious and takes longer to prepare the solvent—we have created and verified a straightforward method that can be used for pharmacokinetics research as well as for the detection and measurement of the real drug in formulation. The obtained formulations were assessed using drug loading percentage, % entrapment efficiency, particle size and zeta potential measurement, and morphological observation. The outcomes demonstrated that the liposomes made using the ethanol injection approach had the finest quality and stability together with excellent potential outcomes. The greatest findings, however, are shown byETNLE 5, which has a zeta potential of roughly 12.7±1.266 mV, a polydispersity index of 0.340±0.051%, and a particle size of 197.3±0.21 nm. The maximum entrapment efficiency (81.78±0.78%) and drug loading (89.62±2.53%) were observed in this batch when compared to the others. % According to an in-vitro drug release investigation, the ETNLE 5 formulation at pH 1.2 and pH 6.4 released 15% and 21% of the medication in the first five minutes, respectively, with a cumulative drug release of 58% and 78%. The improved batch's stability study revealed no appreciable shifts in the assessment parameters. A study on the vitality of A-549 cells using the MTT assay revealed that 200 μM equivalent etoposide liposomes suppress cancer cells more effectively than 64.88% of free medication. These results made it clear how preparation techniques affect cationic liposome performance and how formulation parameters affect entrapment efficiency. They will also serve as a valuable methodological guide for future research on liposome carriers for drug delivery and tumor penetration.

Keywords: Etoposide, Liposome drug delivery system, FTIR spectra, Diffenrtial Scanning Calorimetry (DSC).