Abstract

AMELIORATIVE ROLE OF ANDROGRAPHIS PANICULATA NEES ON CHROMIUM-INDUCED OXIDATIVE STRESS IN LIVER AND LUNGS MITOCHONDRIA

Durga Pada Dolai, Somenath Roy and Sankar Kumar Dey*

ABSTRACT

Mitochondria are the crossroads of several crucial cellular activities; they produce considerable quantities of superoxide radical and hydrogen peroxide, which can damage important macromolecules. Potassium dichromate (K2Cr2O7), a Cr (VI) compound, is the most toxic form of chromium and has been demonstrated to induce toxicity associated with oxidative stress in humans and animals. The aim of this study was to elucidate the protective effects of aqueous extract (AE-Ap) of Andrographis paniculata on chromium-induced oxidative stress in liver and lungs mitochondria. In this investigation, a group of male Wistar rats (100-120 gm) were induced by intraperitonial injection of vehicle (0.9% NaCl), K2Cr2O7 (0.8 mg / 100 g body weight / day), K2Cr2O7 plus AE-AP250 (250 mg/kg body weight/day) and K2Cr2O7 plus AE-AP500 (500 mg/kg body weight/day) for the period of 28 days. Measurement of oxidative stress biomarkers like lipid peroxidation (MDA), conjugated dienes and nitric oxide contents were increased in both liver and lungs mitochondria. The decreased antioxidant marker enzymes like the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (G-S-T) of Cr (VI) treated rats were accompanied by a significant decrease in the levels of glutathione (GSH and GSSG) in liver and lungs mitochondria. The results of the present study suggest that the administration of AE-AP significantly supplement the biochemical marker enzyme, lipid peroxidation and enhanced the antioxidant status in rat’s liver and lungs mitochondria.

Keywords: Chromium, tissues, mitochondria, oxidative stress, Andrographis paniculata Nees.