Abstract

ANTIBACTERIAL PROPERTIES OF SILVER NANOPARTICLES SYNTHESIZED USING LEAF EXTRACT OF ANREDERA CORDIFOLIA AS A REDUCING AGENT

Synodalia C. Wattimena* and Philipus J. Patty

ABSTRACT

In this study, we synthesized silver nanoparticles using leaf extract of Anredera cordifolia as a reducing agent and observed their antibacterial properties against gram positive bacterial strain Staphylococcus aureus and gram negative bacterial strains Escherichia coli. The formation of silver nanoparticles was indicated by the change of colour of the sample after mixing leaf extract and silver nitrate solution and was quantified by measuring the absorbance of light using both colorimeter and UV-VIS. We observed the effect of volume ratio of leaf extract to the silver nitrate solution on the antibacterial properties. We used disc diffusion method to observe the antibacterial properties of silver NPs, which is indicated by their inhibition zones produced by the nanoparticles against the bacteria. We compared the inhibition zones produced by silver nanoparticles prepared with different ratios of leaf extract to silver nitrate solution. For this purpose, we used the ratio of 1:8, 2:7, 1:2 and 1:1. From the results of the measurements and statistical analysis we found that the diameter of inhibition zones produced by silver nanoparticles prepared with the volume ratio of 1:8 is significantly larger than that produced by silver nanoparticles prepared with other ratios. The results apply for both Staphylococcus aureus and Escherichia coli. We also found no significant difference in inhibition zone diameters of Staphylococcus aureus and Escherichia coli, suggesting a broad spectrum antibacterial activity of the Anredera cordifolia leaf extract mediated silver nanoparticles.

Keywords: Antibacterial Properties, Silver Nanoparticles, Leaf Extract, Surface Plasmon Resonance, Inhibition Zones.