Abstract

EXTRACTION OF ACETYLCHOLINE ESTERASE INHIBITORS FROM JASMINUM GRANDIFLORUM FOR THE TREATMENT OF ALZHEIMER’S DISEASE

Darshan S., Brinda V., Hamsa L. and *Guruprasad R.

ABSTRACT

Alzheimer`s disease is the most common dementia contributing to 60-70% of the cases. The widely used treatment for the disease is to suppress the activity of the enzyme acetylcholine esterase which breaks down the neurotransmitter acetylcholine thus, resulting in the deceleration of the disease. This paper briefs about the identification and extraction of acetylcholine esterase inhibitors from the methanolic extract of the plant Jasminum grandiflorum leaves. Zebrafish is used as the model organism to study the effect of plant leaf extract on activity of acetylcholine esterase in both in-vitro and in-vivo conditions. Titrimetric assay which involves the use of an alkali to neutralize the carboxylic acid released by the enzyme is used to determine the enzyme activity in the presence of the plant extract. It was found that the enzyme activity was decreased by 19.97% in-vitro conditions and in in-vivo conditions of diseased fish the enzyme activity was dropped by 21.39%. The methanolic leaf extract was sent to GC-MS studies to determine the organic compounds present in it and this was followed by in-silico studies which revealed that compound cedrol has minimum energy and maximum binding affinity with the acetylcholine esterase enzyme.

Keywords: Alzheimer`s disease, acetylcholine esterase, enzyme activity, docking.