Abstract

SCREENING OF DRUG TARGETING ANGIOGENESIS

Sneha S. Kirgat, Varsha D. Jadhav and Swati R. Dhande*

ABSTRACT

Angiogenesis is the process of formation of new blood vessels from existing one during embryonic development and continues into adult life. Many diseases arise due to altered tissue vascularization and oxygen availability. Insufficient vascular growth contributes to diseases like coronary artery, while excess angiogenesis leads to tumor formation. Thus molecules targeting angiogenesis hold greater potential for treating various diseases. Many herbs from natural origin are currently being investigated to understand their effect on angiogenesis. White Leghorn chick embryo Chorioallontoic membrane (CAM) assay is widely used to study angiogenesis. The aim of present study was to screen various plants for anti-angiogenic and pro angiogenic activity. The plants selected were Parkia biglandulosa (Mimosaceae), Cissus quadrangularis (vitaceae), Solanum surattense (Solanaceae), Bombax ceiba (Bombaceae) and Erythrina variegata (Fabaceae). The chick embryos on embroynal day 8 were exposed to different doses of ethanolic extracts of all plants. On embroynal day 12; visual inspection of CAM showed inhibition of blood vessels formation in ethanolic extracts of Parkia biglandulosa, Solanum surattense and Erythrina variegata treated group indicating its anti-angiogenic activity. While ethanolic extracts of Cissus quadrangularis and Bombax ceiba treated group showed decreased density of blood vessels indicating its pro-angiogenic activity.

Keywords: Angiogenesis, Chorioallantonic Membrane Assay, Parkia Biglandulosa, Cissus Quadrangularis, Solanum Surattense, Bombax Ceiba, Erythrina Variegate.