WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

QUANTITATIVE DETERMINATION OF NIACIN IN AQUEOUS EXTRACT OF OCIMUM SANCTUM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

C. T. Sadashiva*, H. M. Firoz Hussain, S. Nanjundaiah, R. Patil, R. Ashok, M. Sudheer, Y.D.Pavithra and P. Arunagiri

ABSTRACT

Niacin (C6H5NO2), also known as nicotinic acid is one of the precious human nutrient and a form of B3 vitamin group available in herbs. The quantitative estimation of these organic acids in the solvent extract is very crucial and the methodology of the identification and quantification were distinctly different due to its various chemical properties. The present study was designed to evaluate the presence of niacin in the aqueous extract of Ocimum sanctum. L by reverse phase high performance liquid chromatography (RP-HPLC). RP-HPLC, a simple, sensitive and precise methodology has been developed, validated and used for quantitative determination of niacin from the aqueous extract of O. sanctum. L. The RP-HPLC was carried out followed by the aqueous extraction of O. sanctum. The C18 (250mm x 4.6mm, 5 μm) column and a mobile phase comprising of 0.05M Potassium Dihydrogen phosphate acetonitrile (65:35) which was obtained by trial and error method at a flow rate of 0.7 ml /min in an isocratic system. The detection of niacin was carried out at 240 nm. The results were concluded as the higher amount of niacin was present in the aqueous extract of O. sanctum. Further studies are warranted to exhibit the various bio-protective effects of O. sanctum

Keywords: Ocimum sanctum, Niacin, chromatography, RP-HPLC, C18 column.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More