Abstract

IMMERSION VACCINATION OF WHOLE CELL (WC) AND OUTER MEMBRANE PROTEIN (OMP) VACCINES IN LABEO ROHITA FINGERLINGS AGAINST STAPHYLOCOCCOSIS DISEASE

Deepalakshmi R.* and Brindha Devi G. B.

ABSTRACT

Immersion vaccination is a widely accepted and sensible technique for mass vaccination in small fishes (fry and fingerlings). Whole cell (WC) and Outer membrane protein (OMP) vaccines were prepared from the Staphylococcus aureus culture. Qualitative analysis by Lowry’s method and Quantitative analysis by SDS-PAGE of proteins were performed for the prepared vaccines. Fingerlings were immunized after pre-treatment with hyper-osmotic solution of 2% NaCl for 5 minutes by immersion (bath) vaccination of prepared vaccines for about 30 minutes. Immunization was carried out twice as booster dose on day 1 and second dose on day 30. After 30 and 60 dpv fingerlings from each group were bath challenged with virulent Staphylococcus aureus in lethal dose concentration of 1x108 cells/ml. Fingerlings were observed for pathological signs and symptoms. Mortality rate and relative percent survival were recorded for up-to 10 days. After 30 dpv control group showed 100% mortality within 10 days of bacterial challenge, whereas significant relative percent survival (RPS) was recorded in vaccinated groups with 68% in WC group and 64% in OMP group. After 60 dpv there was a significant increase in the RPS rate in OMP group with 84% and 76% in WC.

Keywords: Labeo rohita (rohu), Whole cell vaccine (WC), Outer membrane protein vaccine (OMP), Staphylococcus aureus, Mortality and Relative percent survival (RPS).