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Abstract

ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF ARSENIC RESISTANT BACTERIA (ARB) FROM RIVER GANGA AND GROUND WATER FROM NORTH BIHAR

Prangya Paramita Acharya, Dr. Surajit Das* and Ajay Kumar Sahu

ABSTRACT

12 arsenic resistant strains were isolated from water and sediment samples of river Ganga and ground water from Barauni, North Bihar. The strains BW041, MS133, MS122 showed high resistance towards arsenic and can be used for the bioremediation of arsenic. Out of the 12 isolates 8 were bio film forming. Among them 6 isolates have arcs’ gene which encodes for arsenic reductase enzyme. Arsenate reductase activity was found to be conferred by this arsC gene. The isolates can tolerate wide range of pH and salinity. All the selected isolates gave positive catalyse test and negative for Voger Prosker test. Thus, such metabolically well-equipped bacterial strains with highest As3+ oxidation activities may be used for bioremediation of as contaminated water and effluents in the near future.

Keywords: Arsenic, Bacteria, Bio film, Arsenic reductase.


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