Abstract

ANTIOXIDANT, CYTOTOXICITY AND GENOTOXICITY TESTING OF BETULA PUBESCENS

*Navaneetha Krishnan M., Maleeka Begum S. F. and Sujatha R.

ABSTRACT

About 80% of the population living in rural areas in developing countries depends on traditional medicine for their health care needs. Many of the powerful drugs used in modern medicines originated in plants. Today’s plant-based drugs treat a range of diseases, from headaches to cancer. This paper contains the in vitro study of the Betulaceae family of the plant Betula pubescens. The phytochemical study covers the compounds of the plant extract and the antioxidant activity of the extract. The radical scavenging activity (DPPH) is allowed. The experiment MTT assay is worked on the extract for its cytotoxicity. The Ames test, a preliminary genotoxicity is conducted to know the extract is carcinogen (mutagent) or not for the further medical use. The results evidently showed Betula pubescens has a antioxidant activity, non cytotoxic and genotoxic.

Keywords: Ames Test, MTT assay, DPPH activity, phytochemical study, Betula pubescens.