Abstract

HPTLC METHOD DEVELOPMENT AND VALIDATION OF EPERISON HYDROCHORIDE IN API AND PHARMACEUTICAL DOSAGE FORM

P. G. Suryawanshi and M. S. Phoujdar

ABSTRACT

High-performance thin layer chromatography (HPTLC) is one of the sophisticated instrumental techniques for qualitative and quantitative analysis of drug. A suitable HPTLC method was developed for the identification of API - Eperisone hydrochloride in formulation – Myosone using the stationary phase: TLC Silica gel 60 F254 and mobile phase: Toluene-Methanol (8:2) v/v. After the application of bands using CAMAG Automatic TLC Sampler 4, the plate was developed in the solvent system up to 70mm in CAMAG Twin Trough Chamber. Band was detected at 267nm. The method was validated in terms of linearity, accuracy, precision, LOD-LOQ, reproducibility and specificity according to ICH guidelines.

Keywords: HPTLC, Eperison HCl, Validation, CAMAG, Methanol ,Toluene