Abstract

QUANTITATIVE ESTIMATION OF DEXTRAN CONJUGATED PPI DENDRIMER FOR DELIVERY OF DOXORUBICIN HYDROCHLORIDE AS AN ANTICANCER DRUG

Kumari Manisha*, Dr. Agrawal Amit, Mishra T. S., Kumari Mamta and Bhawarker Swati

ABSTRACT

The major drawbacks associated with anticancer drugs are their toxic effects and non-availability of appropriate dose at the desired site. Conventional mode of administration of such bioactives leads to their interaction with cancerous as well as normal cells. This not only precipitates toxicity but also adds to considerable loss of dose before reaching the desired site. Any attempt to slightly modify the chemical structure of the drug to achieve the results may reduce its efficacy. To avoid the possible hazards in cancer chemotherapy we selected novel dextran conjugated PPI dendritic systems as drug carriers. The drug was identified for any impurities by chemical tests, UV scanning and IR spectroscopy. The absorption maxima of the drug Doxorubicin Hydrochloride in methanol and was found to be 480.2 nm. The various peaks obtained in the FTIR spectrum matched with the IR spectrum. The standard curves of Doxorubicin HCl were prepared in different media and the absorbance data obtained was subjected to linear regression. The equation of line obtained were Y = 0.0161X + 0.0001, Y = 0.0161X - 0.0044, Y = 0.0179X + 0.0017 and the correlation coefficients were found to be 0.9977, 0.9942, 0.9987, respectively for standard curve of drug in water, PBS (pH 7.4) and phosphate buffer (pH 6.4) which are close to 1.0 indicating good linearity. The partition coefficient values obtained were 0.520 in n-octanol:distilled water and 0.441 in n-octanol:PBS (pH 7.4). This concludes that the drug in its salt form is aqueous soluble in nature and can be assayed for various parameters in water as base using the standard curve made in UV range. The outcome of the present study suggests that the macromolecule conjugated dendrimers extended the circulation time and the tumor-specific delivery of the bioactive.

Keywords: DENDRIMERS, PPI, ANTICANCER, UV SPECTROSCOPY, IR SPECTRUM PARTITION COEFFICIENT.