Abstract

ANTIOXIDATIVE POTENTIALS OF COOKED BAMBARA GROUNDNUT BASED DIET (VIGNA SUBTERRANEAN) ON LIPID PEROXIDATION STATUS IN PRETREATED ALLOXAN INDUCED DIABETIC RATS

Ajayi Olubunmi,* Adegbaye, Oluwatosin and Akinyele Dare

ABSTRACT

The present study examines the antioxidant potentials of Cooked Bambara groundnut (Vigna subterranean) based diet in pretreated alloxan induced diabetic rats. Thirty (30) male albino rats (Rattus novergicus) were ramdomly divided into three experimental groups (A, B, C) i.e 10 rats in each group. GroupA received standard formulated diet, GroupB were fed with 50:50 ratio of corn starch and cooked Bambara nut based diet, GroupC received whole cooked Bambara nut based diet for four weeks. After four weeks of feeding, the three groups were sub-divided into subgroups A1, A2, B1, B2 and C1, C2. Subgroups A2, B2 and C2 were induced with 150mg/kg body weight of alloxan by a single intraperitonial injection. Each Subgroups were maintained on their respective diet for two weeks, after which the blood samples were collected via cardiac puncture, heart was removed and assayed for Malondialdehyde (MDA), Reduced glutathione (GSH) and antioxidant enzyme activities (Catalase, Superoxide dismutase). Significant (p < 0.05) elevation in MDA, and a reduction in antioxidant activities were observed in plasma and heart of groupA2 compared with groupA1. A significant decrease in plasma and heart MDA level was observed in groupC2 compared with groupC1. Plasma and heart antioxidant activities of groupC2 significantly increased (P ≤ 0.05) when compared with groupC1. There was no significant difference in plasma and heart MDA level and antioxidant activities in groupB2 compared with groupB1. In conclusion, Cooked Bambara groundnut exhibits its antioxidant potentials by alleviating oxidative stress. However, it appears that increased concentration of cooked Bambara groundnut in the diet could be more effective in preventing oxidative stress in pretreated alloxan induced diabetic rats.

Keywords: Antioxidant, alloxan, oxidative stress, Vigna subterranean.