Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITIES OF METHANOLIC EXTRACT OF CATHARANTHUS ROSEUS LEAVES AGAINST PATHOGENIC STRAINS

S.S.Maithili* and C.Mekala

ABSTRACT

Methanolic extract of leaves of Catharanthus roseus shows antibacterial action against three pathogenic micro-organisms, (Klebsiella pneumonia , Staphylococcus aureus, Escherichia coli ). The antibacterial action of extract is compared with a standard drug (streptomycin) at various concentrations (25, 50, 75, and 100 μg/ml).Before that preliminary phytochemical screening was performed by using a standard chemical test and antibacterial action screened by using Cylinder-plate assay method. Phytochemical analysis of the leaves extract showed the presence of Tannins, Saponins, Steroids, Flavonoids , Glycosides, Anthroquinones , Phenolic compounds .Phytochemical investigations explore active constituents which are very significant in drug development. The study revealed a notable antibacterial inhibitory activity of methanolic extract of the leaves. Molecular characterization of the Catharanthus roseus experiment that leaves produces/ accumulated more amounts of antimicrobial agent against the clinical pathogens. DNA fingerprinting technique had the major advancement in measure of genetic distance . In the present study DNA fingerprinting was used to produce unique fingerprinting, which would giving the clear band patterns.

Keywords: Preliminary phytochemical screening, Catharanthus roseus ,Cylinder-plate assay method, Molecular characterization.